Library preparation

iLab-300R

Fully automatic sample preparation system

A high-throughput automated sample-preparation system on a 30-position deck. 96- and 384-channel heads run NGS library construction for 96 samples or magnetic-bead cleanup for 384 samples in one pass.

Features

30-position open deck compatible with third-party kits
Heads: 96-channel (70/250/1000 μL), 384-channel 70 μL, and 24-channel 1000 μL
96-sample NGS library construction or 384-sample bead cleanup per run
Optional magnetic, thermal, shaking, PCR, and quantification modules
1 μL CV ≤ 5%; XYZ positioning to 0.1 mm

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Introduction

iLab-300R is a high-throughput automated sample-preparation system: a precision pipetting module, contamination control, and a software console. Optional magnetic, thermal, shaking, PCR, and quantification modules attach to the deck.

High-throughput pipetting head

Five heads are available: 96-channel 70 μL, 96-channel 250 μL, 96-channel 1000 μL, 384-channel 70 μL, and 24-channel 1000 μL.

Deck

Thirty open positions hold magnetic, thermal, shaking, PCR, and quantification modules in any layout and accept third-party kits. One loading can run nucleic-acid extraction or library cleanup for 384 samples.

Multifunction arm

A single XYZ arm positions to 0.1 mm on 96- and 384-well plates and grips, moves, and places plates.

Magnetic rack

Ring or column magnetic racks for 96-well plates make bead capture and elution fast. Methods are tuned for large-volume capture and small-volume elution.

Temperature-control module

The thermal module covers 4–95 °C for lysis, bead elution, and cold hold of enzyme reagents. An aluminum adapter speeds ramps and reduces well-to-well temperature error.

Heating and shaking module

Heating and shaking are combined in one module, so both can run at the same time without taking a second deck position.

PCR module

The PCR module is tuned for even well temperatures through heat and cool steps: NGS and long-read library construction, Sanger sequencing, enzyme reactions, and hybridization.

Emergency stop

An emergency stop halts the instrument at any step to protect operators and samples. The interlock engages and clears quickly.

Control software

A graphical console shows component status, arm position, step, and remaining time. Methods can pause and resume at any point; parameters can be set for the protocol.

Graphical interface

The software shows the deck layout while a method runs. Programming is sequential and open; methods can pause and resume after a fault, and parameters can be customized for the application.

Specifications

Parameter	Value
Dimensions	125 cm × 82 cm × 100 cm
Weight	about 200 kg
Deck positions	30
Pipetting
96-channel 70 μL head	0.5–70 μL
96-channel 250 μL head	1–200 μL
96-channel 1000 μL head	10–1000 μL
384-channel 70 μL head	0.5–70 μL
24-channel 1000 μL head	10–1000 μL
Temperature control
Temperature range	4–95 °C
Temperature accuracy	≤ 1 °C
Temperature stability	≤ 0.5 °C
Heating and shaking module
Temperature range	ambient +5 °C to 105 °C
Temperature accuracy	≤ 1 °C
Temperature stability	≤ 0.5 °C
Shaking speed	800–2000 rpm
Quantification
Sample volume	1–20 μL
Sample throughput	8
Assay time	≤ 5 s/run
Concentration range	0.01–120 ng/μL
PCR module
Temperature range	4–99 °C
Average heating rate	≥ 3.5 °C/s
Average cooling rate	≥ 2.0 °C/s
Module temperature precision	≤ 0.2 °C
Temperature accuracy	≤ 0.3 °C
Contamination control	HEPA and UV
Pipetting precision
70 μL head	1 μL CV ≤ 5.0%; 5 μL CV ≤ 3.0%; 25 μL CV ≤ 2.0%; 50 μL CV ≤ 1.0%
Pipetting accuracy
70 μL head	1 μL ± 10%; 5 μL ± 6.0%; 25 μL ± 4.0%; 50 μL ± 2.0%
Pipetting precision
250 μL head	2 μL CV ≤ 5.0%; 100 μL CV ≤ 1.0%; 200 μL CV ≤ 1.0%
Pipetting accuracy
250 μL head	2 μL ± 10%; 100 μL ± 1.5%; 200 μL ± 1.0%
Pipetting precision
1000 μL head	100 μL CV ≤ 0.5%; 500 μL CV ≤ 0.3%; 900 μL CV ≤ 0.3%
Pipetting accuracy
1000 μL head	100 μL ± 1.5%; 500 μL ± 1.0%; 900 μL ± 1.0%